Protocols are all set for the ecotoxicology and monitoring experiments in Spain led by the University of Murcia and the IMDEA Water Institute. The eastern Mediterranean case-study will start in two weeks and will finish end of September. The laboratory experiments will assess the toxicity of antibiotics and metals used in fish farms to marine biofilms and to see the chemical intake and potential side-effects on two marine benthic invertebrate species. The data generated from these studies will be used to refine environmental threshold concentrations for these pollutants as well as to set the boundaries of the impact zone of Mediterranean fish farms.